T cells are an essential part of the immune system. T-cell populations at differentiation states vary in their migratory behavior, cytokine production, and proliferative capacity, as well as effector function. Analysis of co-receptor and cytokine expression profile by single cell RNAseq and flow cytometry provides new information on the differentiation and functional state of T cells. Further studies, in particular of patient material, will determine whether the understanding of the expression profile and functional status of T cell subsets can help to predict disease progression in inflammatory diseases or to improve the therapeutic efficacy of vaccinations or checkpoint inhibition.
- Christina Iwert (cell culture, FACS, CyTOF, Metabolomics, RNASeq, tansmission electron microscopy)
- Christine Appelt (PBMC isolation, MACS, cell culture, flow cytometry)
- Karla Riesterer (Cell biology)
- Killer-like receptors and GPR56 progressive expression defines cytokine production of human CD4+ memory T cells. [Nat Commun. 2019, 10(1)]
- CD96 expression determines the inflammatory potential of IL-9–producing Th9 cells. [PNAS 2018, 115(13)]
- Differences in CD44 Surface Expression Levels and Function Discriminates IL-17 and IFN-γ Producing Helper T Cells. [PLoS One. 2015, 10(11)]